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Surface expression of the macrophage-associated differentiation Ags was assessed by direct immunofluorescence (thick lines). ZK1 cells were incubated with FITC-labeled anti-mouse F4/80 or FITC-labeled anti-mouse CD11b. Mouse IgG2a and IgG2b were used as isotype controls (light lines). Staining of cells with FITC-labeled anti-mouse Ig compared to unstained cells detects the of cells expressing su
Followed the method of Westerfield [1] to partially digest the chorion with Pronase so that the embryos fall out ofDiscussionOur development of an efficient deyolking protocol for early embryos will influence many areas of zebrafish research. Most remarkably, it enabled us to undertakePage 4 of(page number not for citation purposes)BMC Developmental Biology 2006, 6:
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Y of the isolated endothelial cells was confirmed by RT-PCR using primers to detect mRNA expression of mouse Pecam as a positive control and primers to amplify the smooth muscle-specific mRNA expression of mouse Sm22 as a negative control. Mouse primers amplifying transcripts of Flna (F primer 5'-AAGCCC TCTGCAGTTCTATGTTGA T-3' and R primer 5'-GCA AACGTTTCAGCAGACAGGGTT-3'), 18 S (F primer 5'AGATCAA
S after rehydration (usually 10?0 ). Samples were digested overnight at 37 . MALDI-TOF analysis A 1.2 aliquot of the digestion solution was pipetted onto an AnchorChipTM 384/600 target (Bruker Daltonics GmbH, Bremen, Germany) as described [18]: 0.6 of matrix solution (2 mg -Cyano-4-hydroxycinnamic acid [CHCA, Bruker Daltonik GmbH] dissolved in 0.33 ml of 2.5 aqueous TFA and 0.66 ml acetonitr
Dutch Passion heeft zijn cannabiszaden voor de outdoorkweek bijzonder geselecteerd voor het produceren van geharde, robuuste en uiterst krachtige outdoorplanten, die zeer geschikt zijn voordat gematigde klimaatzones. Autoflowering planten zullen na ongeveer 2-4 weken van vegetatieve groei overgaan in de bloei, ongeacht de hoeveelheid helligkeit en ze zullen klaar zijn voor de oogst in slechts 60 d
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